Anticorps Polyclonal de lapin anti-Alpha Galactosidase A

• Phare
• Validé par KD/KO

Alpha Galactosidase A Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15428-1-AP

Synonymes

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha

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Galerie de données de validation

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  WB analysis of HEK-293 using 15428-1-AP

  WB result of GLA antibody (15428-1-AP, 1:600) with si-Control and si-GLA transfected HEK293 cells..

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  WB analysis using 15428-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15428-1-AP

  IP Result of anti-Alpha galactosidase A (IP:15428-1-AP, 3ug; Detection:15428-1-AP 1:1000) with HEK-293 cells lysate 1800ug.

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

15428-1-AP cible Alpha Galactosidase A dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Galactosidase A Protéine recombinante Ag7609
• Nom complet: galactosidase, alpha
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC002689
• Symbole du gène: GLA
• Identification du gène (NCBI): 2717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLA (Alpha-galactosidase A), also named as Melibiase or Agalsidase, belongs to the glycosyl hydrolase 27 family. It catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. The deficient activity of GLA can cause Fabry disease which is an X-linked inborn error of glycosphingolipid metabolis (PMID: 19287194). Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease (PMID: 20398385). In humans, GLA is synthesized as a 50 kDa precursor, which is further processed to a 46 kDa mature form of the protein (PMID: 9883849, 19387866). It also has a homodimer form with the molecular mass of 110 kDa (PMID: 17287429).

Publications

Species	Application	Title
human,mouse	WB,IHC	J Extracell Vesicles; Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders.; Authors - Joaquin Seras-Franzoso ; KO Validated
human		Orphanet J Rare Dis; Deep characterization of the anti-drug antibodies developed in Fabry disease patients, a prospective analysis from the French multicenter cohort FFABRY.; Authors - Wladimir Mauhin

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