Anticorps Polyclonal de lapin anti-GLAST/EAAT1

• Phare
• Validé par KD/KO

GLAST/EAAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(35)

Avis (2)

N° de cat : 20785-1-AP

Synonymes

EAAT1, GLAST, GLAST-1, SLC1A3, Sodium-dependent glutamate/aspartate transporter 1

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Galerie de données de validation

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  WB analysis of Neuro-2a using 20785-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 20785-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20785-1-AP

  IP result of anti-GLAST (IP:20785-1-AP, 4ug; Detection:20785-1-AP 1:500) with mouse brain tissue lysate 3000ug.

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  IHC staining of mouse brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20785-1-AP (GLAST/EAAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20785-1-AP (GLAST/EAAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20785-1-AP (GLAST/EAAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuro-2a using 20785-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using GLAST antibody (20785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Neuro-2a using 20785-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human GLAST (20785-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules C6, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,
• Résultats positifs en FC (Intra): cellules Neuro-2a

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

20785-1-AP cible GLAST/EAAT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Vache
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLAST/EAAT1 Protéine recombinante Ag14177
• Nom complet: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Masse moléculaire calculée: 542 aa, 60 kDa
• Poids moléculaire observé: 50-55 kDa, 90-100 kDa
• Numéro d’acquisition GenBank: BC037310
• Symbole du gène: GLAST
• Identification du gène (NCBI): 6507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su
mouse		Cell Rep; SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice; Authors - Yan Wang
mouse	WB,IF	Cell Death Differ; RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain.; Authors - Qingli Liang
human	WB	J Neuroimmune Pharmacol; Brain-Derived Exosomal CircRNAs in Plasma Serve as Diagnostic Biomarkers for Acute Ischemic Stroke; Authors - Xinli Jiang
mouse	WB	Biochem Pharmacol; Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability; Authors - Yu Zhou
mouse	WB	Neuropharmacology; Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.; Authors - Yun-Long Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph = 6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina and brain at 5dpf

GLAST/EAAT1 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina and brain at 5dpf (Cat no:20785-1-AP)

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - 0.1M Tris-HCl ph9

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina/brain

GLAST/EAAT1 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina/brain (Cat no:20785-1-AP)

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