Anticorps Polyclonal de lapin anti-GLI1

GLI1 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 25733-1-AP

Synonymes

GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1

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Galerie de données de validation

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  IHC staining of human gliomas using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

25733-1-AP cible GLI1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLI1 Protéine recombinante Ag22962
• Nom complet: GLI family zinc finger 1
• Masse moléculaire calculée: 1106 aa, 118 kDa
• Numéro d’acquisition GenBank: BC013000
• Symbole du gène: GLI1
• Identification du gène (NCBI): 2735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human	IF	Oncotarget; Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide.; Authors - Jilian R Melamed
human	WB	Hum Cell; Retraction Note: Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis; Authors - Fan Li
human	WB	Oncol Rep; Combined inhibition of sonic Hedgehog signaling and histone deacetylase is an effective treatment for liver cancer.; Authors - Jingmin Li
human	WB	Am J Transl Res; Involvement of hedgehog signaling in all-trans retinoic acid-mediated suppression of colon cancer; Authors - Yu Xu
		Biomed Environ Sci; Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.; Authors - Qian Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of weak nuclear and punctate cytosolic.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: U87 Human Glioblastoma

GLI1 Antibody Immunofluorescence, validation (1:500 dilution) in U87 Human Glioblastoma (Cat no:25733-1-AP)