Anticorps Polyclonal de lapin anti-GLI2-Specific

• Phare
• Validé par KD/KO

GLI2-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

44

N° de cat : 18989-1-AP

Synonymes

AW546128, GLI Kruppel family member GLI2, Gli2, GLI2-Specific

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Galerie de données de validation

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  WB analysis using 18989-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18989-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18989-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18989-1-AP

  Immunofluorescent analysis of MCF-7 cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu testiculaire humain
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 40 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Informations sur le produit

18989-1-AP cible GLI2-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GLI-Kruppel family member GLI2
• Masse moléculaire calculée: 168 kDa
• Poids moléculaire observé: 167 kDa， 88 kDa
• Numéro d’acquisition GenBank: NM_001081125
• Symbole du gène: Gli2
• Identification du gène (NCBI): 14633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation; Authors - Pei-Rung Wu
human	WB	J Cell Biol; Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.; Authors - Ivona Mateska
human	WB,IF,IHC	Elife; Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.; Authors - Christina R Adams ; KD Validated
mouse	WB	Acta Pharmacol Sin; 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia; Authors - Xiao-Wei Li
human	WB	Cell Mol Life Sci; HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.; Authors - Uma Thanigai Arasu
human,mouse	WB	Front Cell Dev Biol; Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.; Authors - Wanshuang Cao