Anticorps Polyclonal de lapin anti-GLI2-Specific

• Phare
• Validé par KD/KO

GLI2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18989-1-PBS

Synonymes

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

---

Galerie de données de validation

• 

  WB analysis using 18989-1-AP (same clone as 18989-1-PBS)

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 18989-1-AP (same clone as 18989-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 18989-1-AP (same clone as 18989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18989-1-AP (same clone as 18989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 18989-1-AP (same clone as 18989-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

18989-1-PBS cible GLI2-Specific dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GLI family zinc finger 2
• Masse moléculaire calculée: 168 kDa
• Poids moléculaire observé: 167 kDa， 88 kDa
• Numéro d’acquisition GenBank: NM_005270
• Symbole du gène: GLI2
• Identification du gène (NCBI): 14633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

{{ptg:RelatedPrimaryAntibodies}}