Anticorps Polyclonal de lapin anti-GLI3

GLI3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 28272-1-AP

Synonymes

GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS

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Galerie de données de validation

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  WB analysis of HepG2 using 28272-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 28272-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules SKOV-3
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28272-1-AP cible GLI3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLI3 Protéine recombinante Ag28472
• Nom complet: GLI family zinc finger 3
• Masse moléculaire calculée: 170 aa
• Poids moléculaire observé: 170-190 kDa, 80-90 kDa
• Numéro d’acquisition GenBank: NM_000168
• Symbole du gène: GLI3
• Identification du gène (NCBI): 2737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Publications

Species	Application	Title
rat	WB	Bioengineered; All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression; Authors - Quan Hong
human	WB	Medicine (Baltimore); miR-200c targeting GLI3 inhibits cell proliferation and promotes apoptosis in non-small cell lung cancer cells; Authors - Xiangjun Yi

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