Anticorps Recombinant de lapin anti-GLIPR1L1

GLIPR1L1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240594D10

N° de cat : 83584-3-PBS

Synonymes

240594D10, ALKN2972, GLIPR1 like protein 1, GLIPR1-like protein 1, PRO7434

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Galerie de données de validation

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  WB analysis of human testis using 83584-3-RR (same clone as 83584-3-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83584-3-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 83584-3-RR (same clone as 83584-3-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83584-3-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 83584-3-RR (same clone as 83584-3-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83584-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83584-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83584-3-PBS against Human GLIPR1L1 was performed. The affinity constant is 23.3 nM.

• 

  Affinity and Kinetic Characterization of 83584-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

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Informations sur le produit

83584-3-PBS cible GLIPR1L1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GLIPR1L1 Protéine recombinante Ag14852
• Nom complet: GLI pathogenesis-related 1 like 1
• Masse moléculaire calculée: 242 aa, 27 kDa
• Numéro d’acquisition GenBank: BC014603
• Symbole du gène: GLIPR1L1
• Identification du gène (NCBI): 256710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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