Anticorps Polyclonal de lapin anti-GLIS3

GLIS3 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12678-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse kidney using 12678-1-AP (same clone as 12678-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 12678-1-AP (same clone as 12678-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 12678-1-AP (same clone as 12678-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 12678-1-AP (same clone as 12678-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12678-1-PBS cible GLIS3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLIS3 Protéine recombinante Ag3366
• Nom complet: GLIS family zinc finger 3
• Masse moléculaire calculée: 775 aa, 84 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC033899
• Symbole du gène: GLIS3
• Identification du gène (NCBI): 169792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. GLIS3 (gLIS family zinc finger 3), also known as ZNF515 that localizes to the nucleus and contains five C2H2-type zinc fingers. Expressed in a variety of tissues, including kidney, brain, liver, lung, ovary, pancreas, thymus and skeletal muscle, GLIS3 functions as both an activator and a suppressor of transcription, specifically binding the consensus sequence 5'-GACCACCCAC-3' through its C2H2-type zinc fingers. Defects in the gene encoding GLIS3 are a cause of NDH syndrome; a neonatal diabetes that is characterized by congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. There are various isoform of GLIS3 and molecular weight of one isoform is 70 kDa.

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