Anticorps Recombinant de lapin anti-KGA/GAC

• Phare
• Validé par KD/KO

KGA/GAC Recombinant Antibody for WB, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4C20

N° de cat : CL750-81486

Synonymes

GLS, KGA, GAM, GAC, EC:3.5.1.2

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Galerie de données de validation

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  WB analysis using CL750-81486

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81486 (GLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL750-81486

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated KGA/GAC Recombinant antibody (CL750-81486, Clone:4C20)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-81486 cible KGA/GAC dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KGA/GAC Protéine recombinante Ag3505
• Nom complet: glutaminase
• Masse moléculaire calculée: 669 aa, 73 kDa
• Poids moléculaire observé: 58 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC038507
• Symbole du gène: GLS
• Identification du gène (NCBI): 2744
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808). The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

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