Anticorps Polyclonal de lapin anti-GLTSCR2

• Phare
• Validé par KD/KO

GLTSCR2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 27353-1-AP

Synonymes

GLT, GLTSCR2, NOP53, p60, PICT 1, PICT1

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Galerie de données de validation

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  WB analysis using 27353-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27353-1-AP (GLTSCR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 27353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GLTSCR2 antibody (27353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu pancréatique de souris
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

27353-1-AP cible GLTSCR2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLTSCR2 Protéine recombinante Ag26358
• Nom complet: glioma tumor suppressor candidate region gene 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC010095
• Symbole du gène: GLTSCR2
• Identification du gène (NCBI): 29997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing; Authors - Sotaro Miyao ; KD Validated
human		bioRxiv; Nucleolar dynamics are determined by the ordered assembly of the ribosome; Authors - Jessica Sheu-Gruttadauria
human	WB	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden
human	WB	Biochem Biophys Res Commun; Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis; Authors - Yuya Hirooka

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (03-15-2022)

U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: U2OS (Human Osteosarcoma) cells

GLTSCR2 Antibody Immunofluorescence validation (1:200 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:27353-1-AP)

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