Anticorps Monoclonal anti-GLUD1

GLUD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G10D3

N° de cat : 67026-1-PBS

Synonymes

GLUD, GDH1, GDH 1, GDH, EC:1.4.1.3

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Galerie de données de validation

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  WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67026-1-PBS cible GLUD1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GLUD1 Protéine recombinante Ag6179
• Nom complet: glutamate dehydrogenase 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC040132
• Symbole du gène: GLUD1
• Identification du gène (NCBI): 2746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

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