Anticorps Recombinant de lapin anti-GLUT1

GLUT1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6F14

N° de cat : 81463-1-PBS

Synonymes

SLC2A1, 6F14, GLUT-1, SLC2A1,GLUT1

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Galerie de données de validation

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  WB analysis using 81463-1-RR (same clone as 81463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81463-1-RR (SLC2A1,GLUT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81463-1-RR (same clone as 81463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 81463-1-RR (same clone as 81463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81463-1-PBS cible GLUT1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GLUT1 Protéine recombinante Ag16282
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 1
• Masse moléculaire calculée: 492 aa, 54 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC121804
• Symbole du gène: GLUT1
• Identification du gène (NCBI): 6513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).

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