Anticorps Monoclonal anti-GLUT4

GLUT4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G7C9

N° de cat : 66846-1-PBS

Synonymes

SLC2A4, 3G7C9, Glucose transporter GLUT 4, Glucose transporter type 4, insulin-responsive, GLUT 4

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Galerie de données de validation

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  WB analysis using 66846-1-Ig (same clone as 66846-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  WB analysis using 66846-1-Ig (same clone as 66846-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66846-1-Ig (same clone as 66846-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66846-1-Ig (same clone as 66846-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 66846-1-Ig (same clone as 66846-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GLUT4 (66846-1-Ig, Clone:3G7C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66846-1-PBS cible GLUT4 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GLUT4 Protéine recombinante Ag15390
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 4
• Masse moléculaire calculée: 509 aa, 55 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC069615
• Symbole du gène: GLUT4
• Identification du gène (NCBI): 6517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glucose transporter 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4), is a transporter protein regulating glucose transport across cell membranes in an INS-dependent manner.

What is the molecular weight of GLUT4? Is GLUT4 post-translationally modified?

The molecular weight of GLUT4 transporter is 55 kDa. GLUT4 can be N-glycosylated, which is important for its stability and trafficking between the recycling compartment and plasma membrane (PMID: 21757715 and 22545627), and it can also be ubiquitinated and phosphorylated (PMID: 23665900).

What is the subcellular localization of GLUT4?

Glucose transporters, including GLUT4, are multiple-pass integral membrane proteins. GLUT4 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes. The localization of GLUT4 depends on stimulation with  INS - in basal conditions GLUT4 is retained intracellularly, while upon  INS stimulation it is translocated to the plasma membrane (PMID: 18570632).

What molecules can be transported by GLUT4?

Although the main substrate of GLUT4 transport is glucose, it can also transport glucosamine.

What is the tissue expression pattern of GLUT4?

GLUT4 is expressed in white and brown adipose tissue and in muscle and heart cells, where it is the main glucose transporter responsible for peripheral glucose uptake in response to INS.

Which cell lines can be used to study insulin-dependent GLUT4 protein translocation in glucose uptake assays?

Fat and muscle cells are primarily used in glucose uptake assays because they physiologically respond to  INS(PMID: 26646194). The most commonly used cell lines are 3T3-L1 cells (murine pre-adipose fibroblasts), L6 cells (rat myoblasts), and C2C12 cells (murine myoblasts).

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