Anticorps Recombinant de lapin anti-GM-CSF

GM-CSF Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240183C7

N° de cat : 98050-1-PBS

Synonymes

CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2

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Galerie de données de validation

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  FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

  1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG(red). 1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98050-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98050-1-RR against Human GM-CSF were performed. The affinity constant is 3.46 nM.

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Informations sur le produit

98050-1-PBS cible GM-CSF dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: colony stimulating factor 2 (granulocyte-macrophage)
• Symbole du gène: GM-CSF
• Identification du gène (NCBI): 1437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

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