Anticorps Recombinant de lapin anti-GOLGA2/GM130

• Phare
• Validé par KD/KO

GOLGA2/GM130 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1M17

N° de cat : 82705-8-PBS

Synonymes

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, 1M17, GM130 autoantigen

---

Galerie de données de validation

• 

  WB analysis using 82705-8-RR (same clone as 82705-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

  WB result of GOLGA2/GM130 antibody (82705-8-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 82705-8-RR (same clone as 82705-8-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 82705-8-RR (same clone as 82705-8-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82705-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82705-8-RR against Human GM130/GOLGA2 were performed. The affinity constant is 0.942 nM.

---

Informations sur le produit

82705-8-PBS cible GOLGA2/GM130 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GOLGA2/GM130 Protéine recombinante Ag1848
• Nom complet: golgi autoantigen, golgin subfamily a, 2
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC014188
• Symbole du gène: GM130
• Identification du gène (NCBI): 2801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

{{ptg:RelatedPrimaryAntibodies}}