Anticorps Recombinant de lapin anti-GMEB1-Specific

• Phare
• Validé par KD/KO

GMEB1-Specific Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241447C6

N° de cat : 84448-1-RR

Synonymes

GMEB1, 241447C6, DNA-binding protein p96PIF, Glucocorticoid modulatory element-binding protein 1, GMEB 1

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Galerie de données de validation

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  WB analysis of HepG2 using 84448-1-RR

  WB result of GMEB1-Specific antibody (84448-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB1-Specific transfected HepG2 cells.

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  WB analysis using 84448-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84448-1-RR (GMEB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84448-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GMEB1-Specific Recombinant antibody (84448-1-RR, Clone:241447C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Daudi, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84448-1-RR cible GMEB1-Specific dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glucocorticoid modulatory element binding protein 1
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: NM_006582
• Symbole du gène: GMEB1
• Identification du gène (NCBI): 10691
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GMEB1 is a trans-acting factor that binds to glucocorticoid modulatory elements (GME), acting in estrogen receptor-regulated pathway GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to function in hormone-dependent cancer growth. It associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication.

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