Anticorps Polyclonal de lapin anti-GMEB2

GMEB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13314-1-PBS

Synonymes

P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2

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Galerie de données de validation

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  WB analysis of human placenta using 13314-1-AP (same clone as 13314-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 13314-1-AP (same clone as 13314-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 13314-1-AP (same clone as 13314-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 13314-1-AP (same clone as 13314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 13314-1-AP (same clone as 13314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13314-1-AP (same clone as 13314-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GMEB2 antibody (13314-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13314-1-PBS cible GMEB2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GMEB2 Protéine recombinante Ag4157
• Nom complet: glucocorticoid modulatory element binding protein 2
• Masse moléculaire calculée: 530 aa, 56 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC036305
• Symbole du gène: GMEB2
• Identification du gène (NCBI): 26205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GMEB2, also named as Glucocorticoid modulatory element-binding protein 2, is a 530 amino acid protein, which is expressed in peripheral blood lymphocytes and fetal liver. GMEB2 as a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. The calcualted molecular weight of GMEB2 is 56 kDa, but modified GMEB2 is about 65-70 kDa.

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