Anticorps Monoclonal anti-GMEB2

• Phare
• Validé par KD/KO

GMEB2 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H10H8

N° de cat : 68010-1-PBS

Synonymes

DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79

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Galerie de données de validation

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  WB analysis using 68010-1-Ig (same clone as 68010-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68010-1-Ig (same clone as 68010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68010-1-Ig (same clone as 68010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68010-1-Ig (same clone as 68010-1-PBS)

  WB result of GMEB2 antibody (68010-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB2 transfected MCF-7 cells. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68010-1-Ig (same clone as 68010-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 68010-1-Ig (same clone as 68010-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68010-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68010-1-PBS cible GMEB2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GMEB2 Protéine recombinante Ag31393
• Nom complet: glucocorticoid modulatory element binding protein 2
• Masse moléculaire calculée: 530 aa, 56 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC036305
• Symbole du gène: GMEB2
• Identification du gène (NCBI): 26205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GMEB-2 (glucocorticoid modulatory element-binding protein 2), also known as PIF79 (Parvovirus initiation factor p79) or P79PIF, is a DNA-binding protein that plays a crucial role in modulating transcription upon activation by steroid hormones. GMEB-2 is ubiquitously expressed with preferential expression in developmentally important tissues.

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