Anticorps Monoclonal anti-GMF Beta

GMF Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B10A2

N° de cat : 60062-1-PBS

Synonymes

glia maturation factor beta, GMF Beta, GMFB, GMF-beta

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Galerie de données de validation

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  WB analysis of fetal human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 60062-1-Ig (same clone as 60062-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IF Staining of U-87 MG using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using GMF Beta antibody (60062-1-Ig, Clone: 3B10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunofluorescent analysis of Hela cells, using GMFB antibody 60062-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60062-1-PBS cible GMF Beta dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GMF Beta Protéine recombinante Ag1104
• Nom complet: glia maturation factor, beta
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC005359
• Symbole du gène: GMFB
• Identification du gène (NCBI): 2764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.

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