Anticorps Recombinant de lapin anti-GMIP

GMIP Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250483G2

N° de cat : 86101-2-PBS

Synonymes

ARHGAP46, GEM interacting protein, GEM-interacting protein

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Galerie de données de validation

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  WB analysis using 86101-2-RR (same clone as 86101-2-PBS)

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 86101-2-RR (GMIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 86101-2-RR (same clone as 86101-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86101-2-RR (GMIP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 86101-2-RR (same clone as 86101-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GMIP antibody (86101-2-RR, Clone: 250483G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86101-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86101-2-RR against Human GMIP were performed. The affinity constant is 2.02 nM.

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Informations sur le produit

86101-2-PBS cible GMIP dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GMIP Protéine recombinante Ag23239
• Nom complet: GEM interacting protein
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC126436
• Symbole du gène: GMIP
• Identification du gène (NCBI): 51291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.

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