Anticorps Polyclonal de lapin anti-Geminin

• Phare
• Validé par KD/KO

Geminin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

54

N° de cat : 10802-1-AP

Synonymes

Gem, Geminin, GMNN, RP3 369A17.3

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Galerie de données de validation

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  WB analysis using 10802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10802-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10802-1-AP

  WB result of Geminin antibody (10802-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Geminin transfected HEK-293 cells.

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  WB analysis of mouse testis using 10802-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10802-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10802-1-AP

  IP Result of anti-Geminin (IP:10802-1-AP, 4ug; Detection:10802-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

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  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human breast cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10802-1-AP (Geminin antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 10802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10802-1-AP (Geminin antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10802-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 11 publications below
• IF: See 35 publications below
• IP: See 1 publications below

Informations sur le produit

10802-1-AP cible Geminin dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Geminin Protéine recombinante Ag1076
• Nom complet: geminin, DNA replication inhibitor
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 28-29 kDa
• Numéro d’acquisition GenBank: BC005185
• Symbole du gène: GMNN
• Identification du gène (NCBI): 51053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

Publications

Species	Application	Title
mouse	IF	Nature; Whole genomes redefine the mutational landscape of pancreatic cancer.; Authors - Nicola Waddell
human	IF	Cancer Discov; Biomarkers Associating with PARP Inhibitor Benefit in Prostate Cancer in the TOPARP-B Trial.; Authors - Suzanne Carreira
human	IF	Ann Oncol; Association of RAD51 with homologous recombination deficiency (HRD) and clinical outcomes in untreated triple-negative breast cancer (TNBC): analysis of the GeparSixto randomized clinical trial.; Authors - A Llop-Guevara
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	IF	Nat Commun; Homologous recombination deficiency derived from whole-genome sequencing predicts platinum response in triple-negative breast cancers; Authors - Petra Ter Brugge
human	IF	Sci Transl Med; BRCAness, SLFN11, and RB1 loss predict response to topoisomerase I inhibitors in triple-negative breast cancers.; Authors - Florence Coussy

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