Anticorps Monoclonal anti-Geminin

Geminin Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2E3A2

N° de cat : 66566-1-Ig

Synonymes

GMNN, MGORS6, Gem, 2E3A2

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Galerie de données de validation

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  WB analysis using 66566-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66566-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66566-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66566-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66566-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66566-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules L02, cellules NCCIT
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66566-1-Ig cible Geminin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Geminin Protéine recombinante Ag24283
• Nom complet: geminin, DNA replication inhibitor
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005185
• Symbole du gène: GMNN
• Identification du gène (NCBI): 51053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

Publications

Species	Application	Title
rat	WB	Cell Rep; Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex; Authors - Kellen D Winden

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