Anticorps Monoclonal anti-Geminin

Geminin Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E3A2

N° de cat : 66566-1-PBS

Synonymes

GMNN, 2E3A2, Gem, MGORS6

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Galerie de données de validation

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  WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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  WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66566-1-Ig (same clone as 66566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66566-1-Ig (same clone as 66566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51458-1

  Cytometric bead array sample test of MP51458-1, GMNN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66566-1-PBS. Detection antibody: 66566-2-PBS.

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  Cytometric bead array standard curve of MP51458-1

  Cytometric bead array standard curve of MP51458-1, GMNN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66566-1-PBS. Detection antibody: 66566-2-PBS. Standard:Ag24283. Range: 1.563-200 ng/mL.

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Informations sur le produit

66566-1-PBS cible Geminin dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Geminin Protéine recombinante Ag24283
• Nom complet: geminin, DNA replication inhibitor
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005185
• Symbole du gène: GMNN
• Identification du gène (NCBI): 51053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

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