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Anticorps Recombinant de lapin anti-Geminin

Geminin Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240835E6

N° de cat : 84199-1-PBS

Synonymes

GMNN, MGORS6, Gem, 240835E6

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Geminin Recombinant antibody (84199-1-RR)

    IF Staining of HepG2 using 84199-1-RR (same clone as 84199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GMNN antibody (84199-1-RR, Clone: 240835E6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84199-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84199-1-RR

    Affinity and Kinetic Characterization of 84199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84199-1-RR against Human GMNN were performed. The affinity constant is 0.876 nM.

Informations sur le produit

84199-1-PBS cible Geminin dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Geminin Protéine recombinante Ag1076
Nom complet geminin, DNA replication inhibitor
Masse moléculaire calculée 24 kDa
Numéro d’acquisition GenBankBC005185
Symbole du gène GMNN
Identification du gène (NCBI) 51053
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

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