Anticorps Polyclonal de lapin anti-GMPPB

• Phare
• Validé par KD/KO

GMPPB Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15094-1-AP

Synonymes

GMPPB, KIAA1851

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Galerie de données de validation

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  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 15094-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15094-1-AP (GMPPB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

15094-1-AP cible GMPPB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GMPPB Protéine recombinante Ag7168
• Nom complet: GDP-mannose pyrophosphorylase B
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC001141
• Symbole du gène: GMPPB
• Identification du gène (NCBI): 29925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GMPPB is a cytoplasmic enzyme that catalyzes the formation of GDP-mannose, a key substrate for several glycosylation pathways, including O-mannosylation of α-DG. GMPPB facilitates the formation of the sugar donor GDP-mannose and pyrophosphate from mannose-1-phosphate and GTP, a very early step in the glycosylation pathway (PMID: 37834154, 27147698). GMPPB mutations correlate with several congenital diseases, including severe congenital muscular dystrophy (CMD) with abnormalities in the brain and eye (PMID: 26310427, 30684953). Western blot analysis detected GMPPB at an apparent molecular mass of 40 kDa.

Publications

Species	Application	Title
human	WB	iScience; A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.; Authors - Norio Sasaoka
mouse	WB	Front Mol Biosci; Altered Glycosylation in the Aging Heart.; Authors - Patricia Franzka
zebrafish	WB	Mol Biomed; GMPPB-congenital disorders of glycosylation associate with decreased enzymatic activity of GMPPB.; Authors - Zhe Liu
human,mouse	WB	Front Nutr; Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12; Authors - Patricia Franzka
human	WB,IHC	Int J Mol Sci; Silencing GMPPB Inhibits the Proliferation and Invasion of GBM via Hippo/MMP3 Pathways; Authors - Zi-Lu Huang ; KD Validated