Anticorps Polyclonal de lapin anti-GNAO1

GNAO1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 12635-1-AP

Synonymes

G ALPHA o, GNAO, GNAO1

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Galerie de données de validation

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  WB analysis using 12635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12635-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12635-1-AP

  IP Result of anti-GNAO1 (IP:12635-1-AP, 3ug; Detection:12635-1-AP 1:600) with mouse brain tissue lysate 3600ug.

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  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Y79, tissu cérébral de rat, tissu hépatique humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

12635-1-AP cible GNAO1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAO1 Protéine recombinante Ag3330
• Nom complet: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Masse moléculaire calculée: 302 aa, 35 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC030027
• Symbole du gène: GNAO1
• Identification du gène (NCBI): 2775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

Publications

Species	Application	Title
human	WB	Blood; Identification of functional cooperative mutations of GNAO1 in human acute lymphoblastic leukemia.; Authors - Lili Song
human	WB	Neurochem Res; Efficacy of Hybrid Tetrahydrobenzo[d]thiazole Based Aryl Piperazines D-264 and D-301 at D₂ and D₃ Receptors.; Authors - Juan Zhen
human	WB	Gene; DNMT1 mediated promoter methylation of GNAO1 in hepatoma carcinoma cells.; Authors - Diannan Xu
human	IHC	Int J Mol Med; Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis.; Authors - Zhen Liu
human	IHC	Biosci Rep; The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma.; Authors - Pei Xiaoyu X
human	IHC	J Healthc Eng; GNAO1 as a Novel Predictive Biomarker for Late Relapse in Hepatocellular Carcinoma.; Authors - Meiling Du