Anticorps Monoclonal anti-GNAO1

GNAO1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5G2

N° de cat : 68027-1-PBS

Synonymes

DKFZp686O0962, G ALPHA o, GNAO, GNAO1

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Galerie de données de validation

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  WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68027-1-Ig (GNAO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using GNAO1 antibody (68027-1-Ig, Clone: 1B5G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68027-1-PBS cible GNAO1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GNAO1 Protéine recombinante Ag32045
• Nom complet: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Masse moléculaire calculée: 302 aa, 35 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC030027
• Symbole du gène: GNAO1
• Identification du gène (NCBI): 2775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

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