Anticorps Polyclonal de lapin anti-GNAQ

GNAQ Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 27264-1-AP

Synonymes

G ALPHA q, GAQ, GNAQ

---

Galerie de données de validation

• 

  WB analysis of PC-12 using 27264-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27264-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27264-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules PC-12, cellules HepG2, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27264-1-AP cible GNAQ dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAQ Protéine recombinante Ag26170
• Nom complet: guanine nucleotide binding protein (G protein), q polypeptide
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC057777
• Symbole du gène: GNAQ
• Identification du gène (NCBI): 2776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

{{ptg:RelatedPrimaryAntibodies}}