Anticorps Monoclonal anti-GNAQ

• Phare
• Validé par KD/KO

GNAQ Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2H5G6

N° de cat : 68191-1-PBS

Synonymes

2H5G6, CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q

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Galerie de données de validation

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  WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68191-1-Ig (same clone as 68191-1-PBS)

  WB result of GNAQ antibody (68191-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNAQ transfected MCF-7 cells. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 68191-1-Ig (same clone as 68191-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 68191-1-Ig (same clone as 68191-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68191-1-Ig (same clone as 68191-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68191-1-PBS cible GNAQ dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GNAQ Protéine recombinante Ag26178
• Nom complet: guanine nucleotide binding protein (G protein), q polypeptide
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC057777
• Symbole du gène: GNAQ
• Identification du gène (NCBI): 2776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

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