Anticorps Monoclonal anti-GNAS

GNAS Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A9C10

N° de cat : 66253-1-PBS

Synonymes

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

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Galerie de données de validation

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  WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66253-1-Ig (same clone as 66253-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66253-1-PBS cible GNAS dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GNAS Protéine recombinante Ag20530
• Nom complet: GNAS complex locus
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002722
• Symbole du gène: GNAS
• Identification du gène (NCBI): 2778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

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