Anticorps Polyclonal de lapin anti-GNAT1

GNAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55167-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 55167-1-AP (same clone as 55167-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye tissue using 55167-1-AP (same clone as 55167-1-PBS)

  WB results of GNAT1 antibody (55167-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 55167-1-AP (same clone as 55167-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 55167-1-AP (same clone as 55167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 55167-1-AP (same clone as 55167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55167-1-AP (same clone as 55167-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55167-1-AP (GNAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 55167-1-AP (same clone as 55167-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GNAT1 (55167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55167-1-PBS cible GNAT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: NM_000172
• Symbole du gène: GNAT1
• Identification du gène (NCBI): 2779
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.

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