Anticorps Polyclonal de lapin anti-GNB2

GNB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16090-1-PBS

Synonymes

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2

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Galerie de données de validation

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  WB analysis of HeLa using 16090-1-AP (same clone as 16090-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16090-1-AP (same clone as 16090-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16090-1-AP (same clone as 16090-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16090-1-AP (same clone as 16090-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 16090-1-AP (same clone as 16090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 16090-1-AP (same clone as 16090-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16090-1-AP (same clone as 16090-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using GNB2 antibody 16090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16090-1-AP (same clone as 16090-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNB2 antibody (16090-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16090-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16090-1-PBS cible GNB2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNB2 Protéine recombinante Ag9125
• Nom complet: guanine nucleotide binding protein (G protein), beta polypeptide 2
• Masse moléculaire calculée: 340 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC010073
• Symbole du gène: GNB2
• Identification du gène (NCBI): 2783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

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