Anticorps Polyclonal de lapin anti-GNB3

GNB3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10081-1-PBS

Synonymes

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

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Galerie de données de validation

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  WB analysis using 10081-1-AP (same clone as 10081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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  WB analysis using 10081-1-AP (same clone as 10081-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse embryo using 10081-1-AP (same clone as 10081-1-PBS)

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10081-1-AP (same clone as 10081-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (10081-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10081-1-PBS cible GNB3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNB3 Protéine recombinante Ag0127
• Nom complet: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC000115
• Symbole du gène: GNB3
• Identification du gène (NCBI): 2784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

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