Anticorps Polyclonal de lapin anti-GNB3

GNB3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15388-1-AP

Synonymes

GNB3, Transducin beta chain 3

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Galerie de données de validation

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  WB analysis using 15388-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15388-1-AP (GNB3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu ovarien humain, tissu pulmonaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15388-1-AP cible GNB3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNB3 Protéine recombinante Ag7050
• Nom complet: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC002454
• Symbole du gène: GNB3
• Identification du gène (NCBI): 2784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.