Anticorps Monoclonal anti-GNB3

• Phare
• Validé par KD/KO

GNB3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B6E1

N° de cat : 67497-1-PBS

Synonymes

2B6E1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

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Galerie de données de validation

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  WB analysis using 67497-1-Ig (same clone as 67497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

  WB result of GNB3 antibody (67497-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNB3 transfected HepG2 cells. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67497-1-Ig (same clone as 67497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 67497-1-Ig (same clone as 67497-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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  WB analysis of rat retina using 67497-1-Ig (same clone as 67497-1-PBS)

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67497-1-PBS cible GNB3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GNB3 Protéine recombinante Ag7050
• Nom complet: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC002454
• Symbole du gène: GNB3
• Identification du gène (NCBI): 2784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

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