Anticorps Polyclonal de lapin anti-GNB5

• Phare
• Validé par KD/KO

GNB5 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 11045-2-AP

Synonymes

GB5, Gbeta5, GNB5, Transducin beta chain 5

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Galerie de données de validation

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  WB analysis of mouse brain using 11045-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11045-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 11045-2-AP

  IP result of anti-GNB5 (IP:11045-2-AP, 4ug; Detection:11045-2-AP 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11045-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11045-2-AP (GNB5 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

11045-2-AP cible GNB5 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNB5 Protéine recombinante Ag1518
• Nom complet: guanine nucleotide binding protein (G protein), beta 5
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-42 kDa
• Numéro d’acquisition GenBank: BC011671
• Symbole du gène: GNB5
• Identification du gène (NCBI): 10681
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.

Publications

Species	Application	Title
human	WB	Oncogene; The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.; Authors - D Fuchs ; KD Validated
rat	WB	J Cell Mol Med; Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring.; Authors - Wenna Zhang
mouse	WB	Neuropharmacology.; A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.; Authors - Saba LM
mouse	WB, IF	PMC Biophys; Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling.; Authors - Christopher M Tracy
human	WB	FEBS J; PMID: 30719834; Authors - Sang-Min Park ; KD Validated
rat	WB	MedComm (2020); Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing; Authors - Yan Wang

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