Anticorps Polyclonal de lapin anti-GNMT

GNMT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18790-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat liver using 18790-1-AP (same clone as 18790-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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  WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 18790-1-AP (same clone as 18790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18790-1-PBS cible GNMT dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNMT Protéine recombinante Ag4598
• Nom complet: glycine N-methyltransferase
• Masse moléculaire calculée: 295 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC032627
• Symbole du gène: GNMT
• Identification du gène (NCBI): 27232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

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