Anticorps Monoclonal anti-GNMT

GNMT Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5E3

N° de cat : 67294-1-PBS

Synonymes

EC:2.1.1.20, glycine N methyltransferase, Glycine N-methyltransferase

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Galerie de données de validation

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  WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67294-1-Ig (same clone as 67294-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67294-1-Ig (same clone as 67294-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67294-1-Ig (same clone as 67294-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67294-1-PBS cible GNMT dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GNMT Protéine recombinante Ag4598
• Nom complet: glycine N-methyltransferase
• Masse moléculaire calculée: 295 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC032627
• Symbole du gène: GNMT
• Identification du gène (NCBI): 27232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

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