Anticorps Polyclonal de lapin anti-GNPAT

• Phare
• Validé par KD/KO

GNPAT Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 14931-1-AP

Synonymes

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

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Galerie de données de validation

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  WB analysis of HepG2 using 14931-1-AP

  WB result of GNPAT antibody (14931-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNPAT transfected HepG2 cells.

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  WB analysis of HepG2 using 14931-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14931-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14931-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14931-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14931-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14931-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14931-1-AP

  IP Result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HEK-293 transfectées, cellules HeLa, cellules PC-3, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IP: cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

14931-1-AP cible GNPAT dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNPAT Protéine recombinante Ag6735
• Nom complet: glyceronephosphate O-acyltransferase
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 65-69 kDa
• Numéro d’acquisition GenBank: BC000450
• Symbole du gène: GNPAT
• Identification du gène (NCBI): 8443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID:21990100) This antibody recognize the 69 kDa GNPAT protein.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.; Authors - Pierre M Jean Beltran ; KD Validated
mouse	WB	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park
human	IF	Int J Mol Sci; Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors.; Authors - Malin Tordis Meyer
human,hamster	WB	Hum Mutat; Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.; Authors - Itzkovitz Brandon B
mouse,rat	WB,IHC,IF	Front Cell Dev Biol; Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.; Authors - Rémi Karadayi ; KD Validated
human	IF	Histochem Cell Biol; C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.; Authors - Srikanth Karnati