Anticorps Monoclonal anti-GNPAT

GNPAT Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5C10

N° de cat : 66453-1-PBS

Synonymes

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

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Galerie de données de validation

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  WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66453-1-Ig (same clone as 66453-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66453-1-Ig (same clone as 66453-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66453-1-PBS cible GNPAT dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GNPAT Protéine recombinante Ag6740
• Nom complet: glyceronephosphate O-acyltransferase
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 65-69 kDa
• Numéro d’acquisition GenBank: BC000450
• Symbole du gène: GNPAT
• Identification du gène (NCBI): 8443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.

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