Anticorps Polyclonal de lapin anti-GNRHR

GNRHR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 22462-1-AP

Synonymes

GnRH R, GnRH receptor, GNRHR, GNRHR1, GRHR, LHRHR, LRHR

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Galerie de données de validation

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  WB analysis of mouse ovary using 22462-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22462-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pituitary adenoma using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary adenoma using 22462-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu d'adénome hypophysaire humain, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

22462-1-AP cible GNRHR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNRHR Protéine recombinante Ag18005
• Nom complet: GnRH receptor
• Masse moléculaire calculée: 328 aa, 38 kDa
• Poids moléculaire observé: 60 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC113546
• Symbole du gène: GNRHR
• Identification du gène (NCBI): 2798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.

Publications

Species	Application	Title
rat	IHC	Food Chem Toxicol; Prenatal genistein exposure in rats affects pubertal onset and serum reproductive hormone levels in male offsprings via the hypothalamic-pituitary-gonadal axis; Authors - Jing Zi

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