Anticorps Polyclonal de lapin anti-GOLGA2/GM130

• Phare
• Validé par KD/KO

GOLGA2/GM130 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

103

Avis (2)

N° de cat : 11308-1-AP

Synonymes

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 11308-1-AP

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11308-1-AP

  WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 11308-1-AP

  WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.

• 

  WB analysis of human spleen using 11308-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 11308-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11308-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MDCK using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 11308-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GOLGA2/GM130 (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules MCF-7, tissu splénique humain
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HEK-293, cellules HepG2, cellules MDCK
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 57 publications below
• IHC: See 3 publications below
• IF: See 50 publications below

Informations sur le produit

11308-1-AP cible GOLGA2/GM130 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: Humain, singe, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOLGA2/GM130 Protéine recombinante Ag1848
• Nom complet: golgi autoantigen, golgin subfamily a, 2
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC014188
• Symbole du gène: GOLGA2
• Identification du gène (NCBI): 2801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publications

Species	Application	Title
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
		Immunity; The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity; Authors - Tian Chen
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human	WB,IF	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
	IF	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-02-2023)

very good for WB.

• Applications: Western Blot

FH

Stephen (Verified Customer) (10-01-2019)

RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: RPE (human)

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