Anticorps Polyclonal de lapin anti-GOLGA2/GM130

• Phare
• Validé par KD/KO

GOLGA2/GM130 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

Publications(1)

N° de cat : CL555-11308

Synonymes

GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen

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Galerie de données de validation

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  IF Staining of HeLa using CL555-11308

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 GOLGA2/GM130 antibody (CL555-11308) at dilution of 1:200.

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  IF Staining of HeLa using CL555-11308

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 GOLGA2/GM130 antibody (CL555-11308) at dilution of 1:200.

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  FC experiment of HepG2 using CL555-11308

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human GOLGA2/GM130 (CL555-11308) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL555-11308 cible GOLGA2/GM130 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOLGA2/GM130 Protéine recombinante Ag1848
• Nom complet: golgi autoantigen, golgin subfamily a, 2
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC014188
• Symbole du gène: GM130
• Identification du gène (NCBI): 2801
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt-resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publications

Species	Application	Title
Human	IF	Signal Transduct Target Ther; Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells; Authors - Pengju Wang

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