Anticorps Polyclonal de lapin anti-GOLPH3

• Phare
• Validé par KD/KO

GOLPH3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (1)

N° de cat : 19112-1-AP

Synonymes

Coat protein GPP34, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS

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Galerie de données de validation

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  WB analysis using 19112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 19112-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19112-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 19112-1-AP

  IP Result of anti-GOLPH3 (IP:19112-1-AP, 3ug; Detection:19112-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19112-1-AP (GOLPH3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19112-1-AP (GOLPH3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 19112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 19112-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19112-1-AP

  Immunofluorescent analysis of HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu pulmonaire de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

19112-1-AP cible GOLPH3 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOLPH3 Protéine recombinante Ag5443
• Nom complet: golgi phosphoprotein 3 (coat-protein)
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC033725
• Symbole du gène: GOLPH3
• Identification du gène (NCBI): 64083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Theranostics; miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.; Authors - Qing Zhang ; KD Validated
human	WB	Cancer Res; A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma.; Authors - Siqi Wu
human	WB	Cell Death Dis; GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer; Authors - Jun-Wei Song
human	IHC	Front Oncol; FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.; Authors - Li-Na Yu
		Br J Cancer; GOLPH3 is a novel marker of poor prognosis and a potential therapeutic target in human renal cell carcinoma.; Authors - Y Xue
human	WB	J Exp Clin Cancer Res; STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.; Authors - Fan Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-17-2023)

fairly good for IF, but not as clear as the images in its booklet

• Applications: Immunofluorescence

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