Anticorps Monoclonal anti-GOLPH3

• Phare
• Validé par KD/KO

GOLPH3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3H2A5

N° de cat : 67777-1-PBS

Synonymes

MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3

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Galerie de données de validation

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  WB analysis using 67777-1-Ig (same clone as 67777-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67777-1-Ig (GOLPH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

  WB result of GOLPH3 antibody (67777-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLPH3 transfected HeLa cells. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLPH3 antibody (67777-1-Ig, Clone: 3H2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67777-1-PBS cible GOLPH3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GOLPH3 Protéine recombinante Ag5443
• Nom complet: golgi phosphoprotein 3 (coat-protein)
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC033725
• Symbole du gène: GOLPH3
• Identification du gène (NCBI): 64083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signaling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

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