Anticorps Polyclonal de lapin anti-GORAB

• Phare
• Validé par KD/KO

GORAB Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 17798-1-AP

Synonymes

NTKL binding protein 1, N-terminal kinase-like-binding protein 1, hNTKL-BP1, hNTKL BP1, golgin, RAB6 interacting

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Galerie de données de validation

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  WB analysis using 17798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17798-1-AP (GORAB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17798-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17798-1-AP (GORAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

17798-1-AP cible GORAB dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GORAB Protéine recombinante Ag12002
• Nom complet: golgin, RAB6-interacting
• Masse moléculaire calculée: 264aa,28 kDa; 394aa,45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC064945
• Symbole du gène: GORAB
• Identification du gène (NCBI): 92344
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GORAB, also known as Scyl1BP1, is a protein that localizes to the trans-side of the Golgi apparatus and plays a significant role in intracellular trafficking and protein glycosylation. It is composed of a central coiled-coil region that is responsible for Golgi targeting, likely through interactions with small GTPases Rab6 and Arf5. GORAB is considered a member of the golgin family of coiled-coil Golgi proteins, which are involved in vesicle tethering. GORAB has been implicated in several cellular functions. It has been proposed to function as a transcriptional activator for neurite outgrowth, as a modulator of MDM2 ubiquitylation impacting p53 levels and apoptosis, and has been shown to play a role in centriole duplication and ciliogenesis.

Publications

Species	Application	Title
mouse	WB,IF	J Invest Dermatol; Gorab Is Required for Dermal Condensate Cells to Respond to Hedgehog Signals during Hair Follicle Morphogenesis.; Authors - Ying Liu ; KO Validated
mouse	WB,IF	FASEB J; GORAB promotes embryonic lung maturation through antagonizing AKT phosphorylation, versican expression, and mesenchymal cell migration.; Authors - Ying Liu ; KO Validated
human	IF	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
		J Cell Sci; Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex; Authors - Teresa Vitali
human	WB	Mol Vis; Identification and functional analysis of a novel oculocerebrorenal syndrome of Lowe (OCRL) gene variant in two pedigrees with varying phenotypes including isolated congenital cataract.; Authors - Ahmed K Shalaby

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