Anticorps Monoclonal anti-GORASP2

GORASP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1C9A3

Avis (1)

N° de cat : 66627-1-Ig

Synonymes

1C9A3, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6

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Galerie de données de validation

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  WB analysis using 66627-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66627-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66627-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66627-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66627-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66627-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (66627-1-Ig, Clone: 1C9A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NCCIT, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

66627-1-Ig cible GORASP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GORASP2 Protéine recombinante Ag17607
• Nom complet: golgi reassembly stacking protein 2, 55kDa
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC007770
• Symbole du gène: GORASP2
• Identification du gène (NCBI): 26003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.; Authors - Julian Nüchel
human	WB,IF	J Cell Biol; Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.; Authors - Yijun Zhang
human	IF	Front Cell Dev Biol; Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression; Authors - Vittoria Marini
	IF	Cell Death Dis; A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.; Authors - Peng Wang
human	WB	Cell Commun Signal; CHOP upregulation and dysregulation of the mature form of the SNAT2 amino acid transporter in the placentas from small for gestational age newborns; Authors - Emma Barroso
rat	IF	Cell Signal; Targeting CDK1 inhibits Golgi apparatus stress-mediated neuroinflammation and neuronal apoptosis after intracerebral hemorrhage by modulating GRASP55 phosphorylation; Authors - Xiaobo Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-11-2020)

This antibody can be used for detecting the Golgi localisation of endogenous Gorasp2.

• Applications: Immunofluorescence,

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