Anticorps Recombinant de lapin anti-GORASP2

GORASP2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242700C11

N° de cat : 85077-5-PBS

Synonymes

242700C11, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6

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Galerie de données de validation

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  WB analysis using 85077-5-RR (same clone as 85077-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85077-5-RR (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85077-5-RR (same clone as 85077-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85077-5-RR (same clone as 85077-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 85077-5-RR (same clone as 85077-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85077-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85077-5-RR against Human GORASP2 were performed. The affinity constant is 5.33 nM.

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Informations sur le produit

85077-5-PBS cible GORASP2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GORASP2 Protéine recombinante Ag0950
• Nom complet: golgi reassembly stacking protein 2, 55kDa
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC007770
• Symbole du gène: GORASP2
• Identification du gène (NCBI): 26003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

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