Anticorps Monoclonal anti-GOT1

• Phare
• Validé par KD/KO

GOT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, poulet, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5A12E10

N° de cat : 60317-1-PBS

Synonymes

GIG18, EC:2.6.1.1, cCAT, CAT, cAspAT

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Galerie de données de validation

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  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60317-1-Ig (same clone as 60317-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60317-1-PBS cible GOT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, poulet, rat, souris

• Réactivité: Humain, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GOT1 Protéine recombinante Ag7012
• Nom complet: glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 40-46 kDa
• Numéro d’acquisition GenBank: BC000498
• Symbole du gène: GOT1
• Identification du gène (NCBI): 2805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

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