Anticorps Monoclonal anti-GOT2

GOT2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, poulet, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B6C6

Avis (1)

N° de cat : 67738-1-Ig

Synonymes

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

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Galerie de données de validation

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  WB analysis using 67738-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67738-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules A431, cellules HEK-293, cellules HepG2, cerveau de poulet, cerveau de rat, cerveau de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque de rat, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67738-1-Ig cible GOT2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, poulet, rat, souris

• Réactivité: Humain, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GOT2 Protéine recombinante Ag6600
• Nom complet: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 39-40 kDa
• Numéro d’acquisition GenBank: BC000525
• Symbole du gène: GOT2
• Identification du gène (NCBI): 2806
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sampath Kumar (Verified Customer) (02-26-2024)

The mouse GOT2 antibody worked consistently well at 1:6000 dilution on Avian skeletal muscle (pectoralis) homogenates. 6ug of total protein per sample was blotted on 0.2um nitrocellulose and probed with GOT2 primary antibody diluted in 5% milk. Anti-mouse secondary antibody at 1:10000 along with ECL prime provided optimum results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Avian skeletal muscle (Pectoralis)

GOT2 Antibody Western Blot validation (1:5000 dilution) in Avian skeletal muscle (Pectoralis) (Cat no:67738-1-Ig)